Spermatozoa are formed in the testicles under the influence of testosterone, LH and FSH . They are stored in the vasa deferentia. The seminiferous tubules account for approximately 75% of the total testicular mass and are composed of Sertoli cells and spermatogenic cells. Sertoli cells facilitate the maturation of the spermatozoa.
Approximately 5 - 7 % of the total volume of semen is spermatozoa, contributed by the testis. Over one half of the volume is produced in the seminal vesicles, and it is this fraction which provides nutritional support for the spermatozoa. The prostate contributes approximately 20% of the total volume of the semen.
Semen should be collected for analysis by masturbation after a three day period of sexual abstinence. The specimen should be collected in a sterile container such as a sputum or urine collection cup. Ideally, the specimen should be analyzed within 30 minutes of collection. During transport to the laboratory, it is advisable to keep the specimen as close to body temperature as can be maintained.
Volume 2 - 6 ml
liquefaction complete in 20 minutes
pH 7.2 - 8.0
WBC 0 - 1000 / cumm
Total count 60 - 150 million/ ml
Morphology 80 - 90% normal morphology